Membrane tubule formation by banana-shaped proteins with or without transient network structure

Abstract

In living cells, membrane morphology is regulated by various proteins. Many membrane reshaping proteins contain a Bin/Amphiphysin/Rvs (BAR) domain, which consists of a banana-shaped rod. The BAR domain bends the biomembrane along the rod axis and the features of this anisotropic bending have recently been studied. Here, we report on the role of the BAR protein rods in inducing membrane tubulation, using large-scale coarse-grained simulations. We reveal that a small spontaneous side curvature perpendicular to the rod can drastically alter the tubulation dynamics at high protein density, whereas no significant difference is obtained at low density. A percolated network is intermediately formed depending on the side curvature. This network suppresses tubule protrusion, leading to the slow formation of fewer tubules. Thus, the side curvature, which is generated by protein--protein and membrane--protein interactions, plays a significant role in tubulation dynamics. We also find that positive surface tensions and the vesicle membrane curvature can stabilize this network structure by suppressing the tubulation.

8 pages, 5 figures

References in corpus (6)

• Fluid membranes can drive linear aggregation of adsorbed spherical nanoparticles
• Membrane mediated aggregation of curvature inducing nematogens and membrane tubulation
• Budding and vesiculation induced by conical membrane inclusions
• Two- or three-step assembly of banana-shaped proteins coupled with shape transformation of lipid membranes
• Role of disclinations in determining the morphology of deformable fluid interfaces
• Formation of polyhedral vesicles and polygonal membrane tubes induced by banana-shaped proteins

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