Graphene promotes axon elongation through local stall of Nerve Growth Factor signaling endosomes

Abstract

Several works reported increased differentiation of neuronal cells grown on graphene; however, the molecular mechanism driving axon elongation on this material has remained elusive. Here, we study the axonal transport of nerve growth factor (NGF), the neurotrophin supporting development of peripheral neurons, as a key player in the time course of axonal elongation of dorsal root ganglion neurons on graphene. We find that graphene drastically reduces the number of retrogradely transported NGF vesicles in favor of a stalled population in the first two days of culture, in which the boost of axon elongation is observed. This correlates with a mutual charge redistribution, observed via Raman spectroscopy and electrophysiological recordings. Furthermore, ultrastructural analysis indicates a reduced microtubule distance and an elongated axonal topology. Thus, both electrophysiological and structural effects can account for graphene action on neuron development. Unraveling the molecular players underneath this interplay may open new avenues for axon regeneration applications.

References in corpus (8)

• The Raman Fingerprint of Graphene
• Large-Area Synthesis of High-Quality and Uniform Graphene Films on Copper Foils
• Raman spectroscopy as a versatile tool for studying the properties of graphene
• Quantifying defects in graphene via Raman spectroscopy at different excitation energies
• Optical Separation of Mechanical Strain from Charge Doping in Graphene
• Raman spectroscopy as probe of nanometer-scale strain variations in graphene
• Rapid CVD growth of millimetre-sized single crystal graphene using a cold-wall reactor
• Interfacing Graphene-Based Materials With Neural Cells

Cited by in corpus (2)

• Stability of Highly Hydrogenated Monolayer Graphene in Ultra-High Vacuum and in Air
• Copper Vapor Catalyzing Role in the Growth of Graphene