Studies of unicellular micro-organisms Saccharomyces cerevisiae by means of Positron Annihilation Lifetime Spectroscopy
arXiv:1509.00411 · doi:10.1515/nuka-2015-0135
Abstract
Results of Positron Annihilation Lifetime Spectroscopy (PALS) and microscopic studies on simple microorganisms: brewing yeasts are presented. Lifetime of ortho - positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer lived component) for lyophilised and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water - the main component of the cell - affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilised, dried and wet yeasts with best possible resolution were obtained using Inverted Microscopy (IM) and Environmental Scanning Electron Microscopy (ESEM) methods. As a result visible changes to the surface of the cell membrane were observed in ESEM images.
arXiv commentNukleonika (2015)
References in corpus (5)
- Test of a single module of the J-PET scanner based on plastic scintillators
- A novel method for the line-of-response and time-of-flight reconstruction in TOF-PET detectors based on a library of synchronized model signals
- Novel method for hit-position reconstruction using voltage signals in plastic scintillators and its application to Positron Emission Tomography
- Compressive Sensing of Signals Generated in Plastic Scintillators in a Novel J-PET Instrument
- A pilot study of the novel J-PET plastic scintillator with 2-(4-styrylphenyl)benzoxazole as a wavelength shifter
Cited by in corpus (5)
- Positronium imaging with the novel multiphoton PET scanner
- Feasibility study of the positronium imaging with the J-PET tomograph
- Positronium Physics and Biomedical Applications
- Potential for biomedical applications of Positron Annihilation Lifetime Spectroscopy (PALS)
- Status and Prospects of Discrete Symmetries Tests in Positronium Decays with the J-PET Detector