Enzymatic AND Logic Gates Operated Under Conditions Characteristic of Biomedical Applications

Abstract

Experimental and theoretical analyses of the lactate dehydrogenase and glutathione reductase based enzymatic AND logic gates in which the enzymes and their substrates serve as logic inputs are performed. These two systems are examples of the novel, previously unexplored, class of biochemical logic gates that illustrate potential biomedical applications of biochemical logic. They are characterized by input concentrations at logic 0 and 1 states corresponding to normal and abnormal physiological conditions. Our analysis shows that the logic gates under investigation have similar noise characteristics. Both significantly amplify random noise present in inputs, however we establish that for realistic widths of the input noise distributions, it is still possible to differentiate between the logic 0 and 1 states of the output. This indicates that reliable detection of abnormal biomedical conditions is indeed possible with such enzyme-logic systems.

arXiv commentPDF, 29 pages

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• Biomolecular Filters for Improved Separation of Output Signals in Enzyme Logic Systems Applied to Biomedical Analysis
• Enzymatic AND Logic Gate with Sigmoid Response Induced by Photochemically Controlled Oxidation of the Output
• Control of Noise in Chemical and Biochemical Information Processing
• Realization and Properties of Biochemical-Computing Biocatalytic XOR Gate Based on Enzyme Inhibition by a Substrate
• Design of Digital Response in Enzyme-Based Bioanalytical Systems for Information Processing Applications
• Can bio-inspired information processing steps be realized as synthetic biochemical processes?
• Modeling and Modifying Response of Biochemical Processes for Biocomputing and Biosensing Signal Processing
• Theoretical Modeling Expressions for Networked Enzymatic Signal Processing Steps as Logic Gates Optimized by Filtering