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10 papers · 1 filter
Gene induction during differentiation of human monocytes into dendritic cells: an integrated study at the RNA and protein levels
C. Angénieux, D. Fricker, J. M. Strub +7
Changes in gene expression occurring during differentiation of human monocytes into dendritic cells were studied at the RNA and protein levels. These studies showed the induction o…
Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients
T. Rabilloud, C. Adessi, A. Giraudel +1
Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradient…
Improved mass spectrometry compatibility is afforded by ammoniacal silver staining
Mireille Chevallet, Hélène Diemer, Sylvie Luche +3
Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based pr…
High expression of antioxidant proteins in dendritic cells: possible implications in atherosclerosis
Aymeric Rivollier, Laure Perrin-Cocon, Sylvie Luche +8
Dendritic cells (DCs) display the unique ability to activate naive T cells and to initiate primary T cell responses revealed in DC-T cell alloreactions. DCs frequently operate unde…
Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view
Mireille Chevallet, Pierre Lescuyer, Hélène Diemer +3
The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which…
Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses
Anita Scheuber, Rachel Rudge, Lydia Danglot +4
Synaptic vesicle (SV) exocytosis mediating neurotransmitter release occurs spontaneously at low intraterminal calcium concentrations and is stimulated by a rise in intracellular ca…