204 citations · 763 across the 45 of their papers we have counts for
9 papers · 1 filter
RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity
Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd +2
The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous recombination to resolve dou…
The End Restraint Method for Mechanically Perturbing Nucleic Acids in silico
Jack W Shepherd, Mark C Leake
Far from being a passive information store, the genome is a mechanically dynamic and diverse system in which torsion and tension fluctuate and combine to determine structure and he…
Single-molecular quantification of flowering control proteins within nuclear condensates in live whole Arabidopsis root
Alex L. Payne-Dwyer, Mark C. Leake
Here we describe the coupled standardisation of two complementary fluorescence imaging techniques and apply it to liquid-liquid phase separated condensates formed from an EGFP fluo…
Investigating molecular crowding during cell division in budding yeast with FRET
Sarah Lecinski, Jack W Shepherd, Lewis Frame +3
Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in th…
Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness
Xin Jin, Ji-Eun Lee, Charley Schaefer +10
Liquid-liquid phase separation is emerging as a crucial phenomenon in several fundamental cell processes. A range of eukaryotic systems exhibit liquid condensates. However, their f…
Correlative approaches in single-molecule biophysics: a review of the progress in methods and applications
Mark C Leake
Here, we discuss a collection of cutting-edge techniques and applications in use today by some of the leading experts in the field of correlative approaches in single-molecule biop…