204 citations · 763 across the 45 of their papers we have counts for
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An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy
Adam J. M. Wollman, Helen Miller, Simon Foster +1
Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image anal…
Single molecule narrowfield microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells
Adam J. M. Wollman, Mark C. Leake
Single-molecule narrowfield microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we desc…
Using Fluorescence Recovery After Photobleaching (FRAP) to study dynamics of the Structural Maintenance of Chromosome (SMC) complex in vivo
Anjana Badrinarayanan, Mark C. Leake
The SMC complex, MukBEF, is important for chromosome organization and segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct spots (or 'foci') in the cell, whe…
Single-molecule observation of DNA replication repair pathways in E. coli
Adam J. M. Wollman, Aisha H. Syeda, Peter McGlynn +1
The method of action of many antibiotics is to interfere with DNA replication - quinolones trap DNA gyrase and topoisomerase proteins onto DNA while metronidazole causes single and…
Force spectroscopy in studying infection
Zhaokun Zhou, Mark C. Leake
Biophysical force spectroscopy tools - for example optical tweezers, magnetic tweezers, atomic force microscopy, - have been used to study elastic, mechanical, conformational and d…
Designing a single-molecule biophysics tool for characterizing DNA damage for techniques that kill infectious pathogens through DNA damage effects
Helen Miller, Adam J. M. Wollman, Mark C. Leake
Antibiotics such as the quinolones and fluoroquinolones kill bacterial pathogens ultimately through DNA damage. They target the essential type IIA topoisomerases in bacteria by sta…