High speed optical holography of retinal blood flow

Abstract

We performed non-invasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate of 39 kHz with a high throughput camera. Local Doppler contrasts emerged from the envelopes of short-time Fourier transforms and the phase of autocorrelation functions of holograms rendered by Fresnel transformation. This approach permitted imaging of blood flow in large retinal vessels (30 microns diameter) over 400 by 400 pixels with a spatial resolution of 8 microns and a temporal resolution of 6.5 ms.

arXiv comment4 pages, 5 figures, 4 links

References in corpus (2)

• Holographic laser Doppler imaging of microvascular blood flow
• Holographic laser Doppler ophthalmoscopy

Cited by in corpus (5)

• In vivo laser Doppler holography of the human retina
• Choroidal vasculature imaging with laser Doppler holography
• Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography
• Reverse contrast laser Doppler holography for lower frame rate retinal and choroidal blood flow imaging
• Laser Doppler holography of the anterior segment for blood flow imaging, eye tracking, and transparency assessment