Holographic laser Doppler imaging of microvascular blood flow
arXiv:1412.0580 · doi:10.1364/JOSAA.31.002723
Abstract
We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency shifted reference beam, permits frequency selective imaging in the radiofrequency range. These Doppler images are acquired with an off axis Mach Zehnder interferometer. Microvascular hemodynamic components mapping is performed in the cerebral cortex of the mouse and the eye fundus of the rat with near-infrared laser light without any exogenous marker. These measures are made from a basic inverse method analysis of local first order optical fluctuation spectra at low radiofrequencies, from 0 Hz to 100 kHz. Local quadratic velocity is derived from Doppler broadenings induced by fluid flows, with elementary diffusing wave spectroscopy formalism in backscattering configuration. We demonstrate quadratic mean velocity assessment in the 0.1 to 10 millimeters per second range in vitro and imaging of superficial blood perfusion with a spatial resolution of about 10 micrometers in rodent models of cortical and retinal blood flow.
15 pages
References in corpus (8)
- Speckle-visibility spectroscopy: A tool to study time-varying dynamics
- Quantitative modeling of laser speckle imaging
- Digital holography with ultimate sensitivity
- Holographic laser Doppler ophthalmoscopy
- Laser Doppler holographic microscopy in transmission: application to fish embryo imaging
- Spatiotemporal heterodyne detection
- Heterodyne interferometer with unequal path lengths
- Parallel heterodyne detection of dynamic light scattering spectra from gold nanoparticles diffusing in viscous fluids