Spatial fluctuations at vertices of epithelial layers: quantification of regulation by Rho pathway

Abstract

In living matter, shape fluctuations induced by acto-myosin are usually studied in vitro via reconstituted gels, whose properties are controlled by changing the concentrations of actin, myosin and cross-linkers. Such an approach deliberately avoids to consider the complexity of biochemical signaling inherent to living systems. Acto-myosin activity inside living cells is mainly regulated by the Rho signaling pathway which is composed of multiple layers of coupled activators and inhibitors. We investigate how such a pathway controls the dynamics of confluent epithelial tissues by tracking the displacements of the junction points between cells. Using a phenomenological model to analyze the vertex fluctuations, we rationalize the effects of different Rho signaling targets on the emergent tissue activity by quantifying the effective diffusion coefficient, the persistence time and persistence length of the fluctuations. Our results reveal an unanticipated correlation between layers of activation/inhibition and spatial fluctuations within tissues. Overall, this work connects the regulation via biochemical signaling with mesoscopic spatial fluctuations, with potential application to the study of structural rearrangements in epithelial tissues.

arXiv comment8 pages, 3 figures

References in corpus (12)

• A density-independent glass transition in biological tissues
• Motility-driven glass and jamming transitions in biological tissues
• Equality connecting energy dissipation with violation of fluctuation-response relation
• Aspiration of biological viscoelastic drops
• Energy barriers govern glassy dynamics in tissues
• Cell adhesion and cortex contractility determine cell patterning in the Drosophila retina
• Effective Temperature of Red Blood Cell Membrane Fluctuations
• Complementarity relation for irreversible process derived from stochastic energetics
• Activity driven fluctuations in living cells
• Coupling biochemistry and mechanics in cell adhesion: a model for inhomogeneous stress fiber contraction
• Nonequilibrium dissipation in living oocytes
• Energetics of active fluctuations in living cells

Cited by in corpus (5)

• Time-(ir)reversibility in active matter: from micro to macro
• Dynamics and escape of active particles in a harmonic trap
• Softness, anomalous dynamics, and fractal-like energy landscape in model cell tissues
• Signatures of motor susceptibility in the dynamics of a tracer particle in an active gel
• Mesoscopic non-equilibrium measures can reveal intrinsic features of the active driving