Tracking single particles on supported lipid membranes: multi-mobility diffusion and nanoscopic confinement
arXiv:1312.6736 · doi:10.1021/jp412203t
Abstract
Supported lipid bilayers have been studied intensively over the past two decades. In this work, we study the diffusion of single gold nanoparticles (GNPs) with diameter of 20 nm attached to GM1 ganglioside or DOPE lipids at different concentrations in supported DOPC bilayers. The indefinite photostability of GNPs combined with the high sensitivity of interferometric scattering microscopy (iSCAT) allows us to achieve 1.9 nm spatial precision at 1 ms temporal resolution, while maintaining long recording times. Our trajectories visualize strong transient confinements within domains as small as 20 nm, and the statistical analysis of the data reveals multiple mobilities and deviations from normal diffusion. We present a detailed analysis of our findings and provide interpretations regarding the effect of the supporting substrate and GM1 clustering. We also comment on the use of high-speed iSCAT for investigating diffusion of lipids, proteins or viruses in lipid membranes with unprecedented spatial and temporal resolution.
References in corpus (2)
Cited by in corpus (7)
- Fast, label-free tracking of single viruses and weakly scattering nanoparticles in a nano-fluidic optical fiber
- Light Microscopy: An ongoing contemporary revolution
- Tracking Brownian motion in three dimensions and characterization of individual nanoparticles using a fiber-based high-finesse microcavity
- Experimental creation and characterization of random potential energy landscapes exploiting speckle patterns
- Multiscale Modeling and Analysis for High-fidelity Interferometric Scattering Microscopy
- Colloids Exposed to Random Potential Energy Landscapes: from Particle Number Density to Particle-Potential and Particle-Particle Interactions
- Tracking Single Particles using Surface Plasmon Leakage Radiation Speckle