Single NanoParticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells

Abstract

Tracking individual nano-objets in live cells during arbitrary long times is an ubiquitous need in modern biology. We present here a method for tracking individual 5 nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against Single fluorescent Molecule Tracking in live COS7 cells on subsecond time scales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. SNaPT has the unique potential to record arbitrary long trajectory of membrane proteins using non-fluorescent nanometer sized labels.

References in corpus (3)

• Direct imaging of lateral movements of AMPA receptors inside synapses
• Photothermal heterodyne imaging of individual nonfluorescent nanoclusters and nanocrystals
• Single metallic nanoparticle imaging for protein detection in cells

Cited by in corpus (8)

• A highly specific gold nanoprobe for live-cell single-molecule imaging
• Light Microscopy: An ongoing contemporary revolution
• Rotational hot Brownian motion
• Small Gold Nanorods with Tunable Absorption for Photothermal Microscopy in Cells
• Nanoscale Thermotropic Phase Transitions Enhance Photothermal Microscopy Signals
• Background-free 3D nanometric localisation and sub-nm asymmetry detection of single plasmonic nanoparticles by four-wave mixing interferometry with optical vortices
• Experimental determination of distance and orientation of metallic nanodimers by polarization dependent plasmon coupling
• Holographic microscopy for the three-dimensional exploration of light scattering from gold nanomarkers in biological media