Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

Abstract

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments.

References in corpus (5)

• Single metallic nanoparticle imaging for protein detection in cells
• Single NanoParticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells
• Digital holography with ultimate sensitivity
• Heterodyne holographic microscopy of gold particles
• Noise and aliases in off-axis and phase-shifting holography

Cited by in corpus (9)

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• (DH) Noise and Signal scaling factors in Digital Holography in week illumination: relationship with Shot Noise
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• Holographic microscopy reconstruction in both object and image half spaces with undistorted 3D grid
• Heterodyne Holography with full control of both signal and reference arms
• Signal and Noise scaling factors in digital holography
• Fish embryo multimodal imaging by laser Doppler digital holography
• Holographic microscopy for the three-dimensional exploration of light scattering from gold nanomarkers in biological media