OpenSPIM - an open access platform for light sheet microscopy

Abstract

Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware and software platform is shared with the scientific community through a public website, thereby making light sheet microscopy accessible for widespread use and optimization to various applications.

7 pages, 3 figures, 6 supplementary videos, submitted to Nature Methods, associated public website http://openspim.org

Cited by in corpus (11)

• Efficient Bayesian-based Multi-View Deconvolution
• Single-molecule techniques in biophysics: a review of the progress in methods and applications
• A one-piece 3D printed flexure translation stage for open-source microscopy
• Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging
• Dynamics of a $Volvox$ Embryo Turning Itself Inside Out
• Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging
• Deep Imaging in Scattering Media with Single Photon Selective Plane Illumination Microscopy (SPIM)
• Axially Overlapped Multi-Focus Light Sheet with Enlarged Field of View
• Simple and open 4f Koehler transmitted illumination system for low-cost microscopic imaging and teaching
• An automated workflow for parallel processing of large multiview SPIM recordings
• Mechanics and Variability of Cell Sheet Folding in the Embryonic Inversion of $Volvox$