Measurement of the Phase Diagram of DNA Unzipping in the Temperature- Force Plane
arXiv:cond-mat/0310633 · doi:10.1103/PhysRevLett.93.078101
Abstract
We separate double stranded lambda phage DNA by applying a fixed force at a constant temperature ranging from 15C to 50C, and measure the minimum force required to separate the two strands, providing the first experimental determination of the phase boundary between single stranded DNA and double stranded DNA in the temperature- force plane. The measurements also offer information on the free energy of dsDNA at temperatures where dsDNA does not thermally denature in the absence of force. While parts of the phase diagram can be explained using existing models and free energy parameters, others deviate significantly. Possible reasons for the deviations between theory and experiment are considered.
17 pages, 2 figures
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Cited by in corpus (49)
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- Inferring DNA sequences from mechanical unzipping data: the large-bandwidth case
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- Thermodynamic relations for DNA phase transitions
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- PT-Symmetry Breaking Transitions in Polymeric Systems
- Unzipping of a double-stranded block copolymer DNA by a periodic force
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- Dynamical modelling of molecular constructions and setups for DNA unzipping
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