Multi-Spectral Reflection Matrix for Ultra-Fast 3D Label-Free Microscopy
arXiv:2309.10951 · doi:10.1038/s41566-024-01479-y
Abstract
Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit the penetration depth. Multi-conjugate adaptive optics and time-gated matrix approaches have been developed to compensate for aberrations but the associated frame rate is extremely limited for 3D imaging. Here we develop a multi-spectral matrix approach to solve these fundamental problems. Based on a sparse illumination scheme and an interferometric measurement of the reflected wave-field at multiple wavelengths, the focusing process can be optimized in post-processing for any voxel by addressing independently each frequency component of the reflection matrix. A proof-of-concept experiment demonstrates the three-dimensional image of an opaque human cornea over a 0.1 mm-field-of-view at a 290 nm-resolution and a 1 Hz-frame rate. This work paves the way towards a fully-digital microscope allowing real-time, in-vivo, quantitative and deep inspection of tissues.
55 pages, 9 figures
References in corpus (9)
- Measuring the Transmission Matrix in Optics : An Approach to the Study and Control of Light Propagation in Disordered Media
- Translation correlations in anisotropically scattering media
- Smart optical coherence tomography for ultra-deep imaging through highly scattering media
- Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
- Exploiting the Time-Reversal Operator for Adaptive Optics, Selective Focusing and Scattering Pattern Analysis
- Distortion matrix concept for deep optical imaging in scattering media
- Three-Dimensional Ultrasound Matrix Imaging
- Multiple scattering limit in optical microscopy
- Optical phase modulation by natural eye movements: application to time-domain FF-OCT image retrieval