Phase separation and dynamical arrest of protein solutions dominated by short-range attractions
arXiv:2209.14663 · doi:10.1063/5.0128643
Abstract
The interplay of phase separation and dynamical arrest can lead to the formation of gels and glasses, which is relevant for such diverse fields as hard and soft condensed matter physics, materials science, food engineering and pharmaceutical industry. Here, the non-equilibrium states as well as the interactions of globular proteins are analyzed. Lysozyme in brine is chosen as a model system with short-range attractions. The metastable gas-liquid binodal and the dynamical arrest line as well as the second virial coefficient have been determined for various solution conditions by cloud-point measurements, optical microscopy, centrifugation experiments and light scattering. If temperature is expressed in terms of , the binodals obtained under various conditions fall onto a master curve, as suggested by the extended law of corresponding states. Arrest lines for different salt concentrations overlap within experimental errors, whereas they do not overlap if the temperature axis is replaced by . This indicates that the binodals are not sensitive to the details of the potential, but can be described by one integral parameter, i.e. , whereas the arrest line appears governed by its attractive part. The experimental findings are supported by numerical results of the non-equilibrium self-consistent generalized Langevin equation theory.
References in corpus (29)
- Colloidal Gels: Equilibrium and Non-Equilibrium Routes
- Extended corresponding-states behavior for particles with variable range attractions
- A Materials Perspective on Casimir and van der Waals Interactions
- Reentrant glass transition in a colloid-polymer mixture with depletion attractions
- Mode-Coupling Theory of the Glass Transition: A Primer
- Interplay between Spinodal Decomposition and Glass Formation in Proteins Exhibiting Short-Range Attractions
- Glasslike Arrest in Spinodal Decomposition as a Route to Colloidal Gelation
- Phase equilibria and glass transition in colloidal systems with short-ranged attractive interactions. Application to protein crystallization
- Scaling of dynamics with the range of interaction in short-range attractive colloids
- A cluster mode-coupling approach to weak gelation in attractive colloids
- A simple patchy colloid model for the phase behavior of lysozyme dispersions
- Confocal microscopy of colloidal particles: towards reliable, optimum coordinates
- A closer look at arrested spinodal decomposition in protein solutions
- Theory and simulation of gelation, arrest and yielding in attracting colloids
- Extended law of corresponding states for protein solutions
- A New Theory of Dynamic Arrest in Colloidal Mixtures
- Phase separation and dynamical arrest for particles interacting with mixed potentials--the case of globular proteins revisited
- General Non-equilibrium Theory of Colloid Dynamics
- Aging of a Homogeneously Quenched Colloidal Glass-forming Liquid
- Systematic characterization of thermodynamic and dynamical phase behavior in systems with short-ranged attraction
- Real Space Analysis of Colloidal Gels: Triumphs, Challenges and Future Directions
- Equilibration of Concentrated Hard Sphere Fluids
- Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments
- Glassy dynamics in asymmetric binary mixtures of hard-spheres
- Equilibration and aging of dense soft-sphere glass-forming liquids
- Thermodynamic properties of short-range attractive Yukawa fluid: Simulation and theory
- Interactions in protein solutions close to liquid-liquid phase separation: Ethanol reduces attractions via changes of the dielectric solution properties
- Non-equilibrium Kinetics of the Transformation of Liquids into Physical Gels
- Universal effective interactions of globular proteins close to liquid-liquid phase separation: corresponding-states behavior reflected in the structure factor