A simple patchy colloid model for the phase behavior of lysozyme dispersions

Abstract

We propose a minimal model for spherical proteins with aeolotopic pair interactions to describe the equilibrium phase behavior of lysozyme. The repulsive screened Coulomb interactions between the particles are taken into account assuming that the net charges are smeared out homogeneously over the spherical protein surfaces. We incorporate attractive surface patches, with the interactions between patches on different spheres modeled by an attractive Yukawa potential. The parameters entering the attractive Yukawa potential part are determined using information on the experimentally accessed gas-liquid-like critical point. The Helmholtz free energy of the fluid and solid phases is calculated using second-order thermodynamic perturbation theory. Our predictions for the solubility curve are in fair agreement with experimental data. In addition, we present new experimental data for the gas-liquid coexistence curves at various salt concentrations and compare these with our model calculations. In agreement with earlier findings, we observe that the strength and the range of the attractive potential part only weakly depend on the salt content.

References in corpus (11)

• Phase diagram of patchy colloids: towards empty liquids
• Interplay between Spinodal Decomposition and Glass Formation in Proteins Exhibiting Short-Range Attractions
• Reversible self-assembly of patchy particles into monodisperse icosahedral clusters
• Phase behavior of a fluid with competing attractive and repulsive interactions
• Controlling crystallization and its absence: Proteins, colloids and patchy models
• Modeling Equilibrium Clusters in Lysozyme Solutions
• On the possibility of extending the Nore-Frenkel generalized law of correspondent states to non-isotropic patchy interactions
• A model colloidal fluid with competing interactions: bulk and interfacial properties
• Patchy sticky hard spheres: analytical study and Monte Carlo simulations
• Fluid-crystal coexistence for proteins and inorganic nanocolloids: dependence on ionic strength
• Collective diffusion coefficient of proteins with hydrodynamic, electrostatic and adhesive interactions

Cited by in corpus (32)

• Interplay of pH and Binding of Multivalent Metal Ions: Charge Inversion and Reentrant Condensation in Protein Solutions
• Effects of patch size and number within a simple model of patchy colloids
• Thermodynamically Driven Assemblies and Liquid-liquid Phase Separations in Biology
• Viscosity and Diffusion: Crowding and Salt Effects in Protein Solutions
• The stability of a crystal with diamond structure for patchy particles with tetrahedral symmetry
• Control of pathways and yields of protein crystallization through the interplay of nonspecific and specific attractions
• A closer look at arrested spinodal decomposition in protein solutions
• Limiting the valence: advancements and new perspectives on patchy colloids, soft functionalized nanoparticles and biomolecules
• Extended law of corresponding states for protein solutions
• The crystallization of asymmetric patchy models for globular proteins in solution
• Budding and vesiculation induced by conical membrane inclusions
• Soft Matter Perspective on Protein Crystal Assembly
• Phase separation and dynamical arrest for particles interacting with mixed potentials--the case of globular proteins revisited
• Characterizing protein crystal contacts and their role in crystallization: rubredoxin as a case study
• The physics of Empty Liquids: from Patchy particles to Water
• Phase diagram and structural properties of a simple model for one-patch particles
• Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments
• Multivalent Ion-Activated Protein Adsorption Reflecting Bulk Reentrant Behavior
• Interactions in protein solutions close to liquid-liquid phase separation: Ethanol reduces attractions via changes of the dielectric solution properties
• How to model the interaction of charged Janus particles
• Fluid-Fluid and Fluid-Solid transitions in the Kern-Frenkel model from Barker-Henderson thermodynamic perturbation theory
• Unexpected decoupling of stretching and bending modes in protein gels
• Electrokinetic and hydrodynamic properties of charged-particles systems: From small electrolyte ions to large colloids
• Universal effective interactions of globular proteins close to liquid-liquid phase separation: corresponding-states behavior reflected in the structure factor
• Filamentous phages as building blocks for reconfigurable and hierarchical self-assembly
• Phase separation and dynamical arrest of protein solutions dominated by short-range attractions
• Protein solutions close to liquid-liquid phase separation exhibit a universal osmotic equation of state and dynamical behavior
• Fluid of fused spheres as a model for protein solution
• Local orientational ordering in fluids of spherical molecules with dipolar-like anisotropic adhesion
• Phase diagram of two-patch colloids with competing anisotropic and isotropic interactions
• Using schematic models to understand the microscopic basis for inverted solubility in $\gamma$D-crystallin
• Theoretical study of phase behaviour of DLVO model for lysozyme and $\gamma$-crystalline aqueous electrolyte solutions