Single-molecule orientation localization microscopy II: a performance comparison

Abstract

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background ratio in typical single-molecule experiments, it is essential to choose an imaging system optimized for the specific target sample. In this work, we compare the performance of multiple state-of-the-art and commonly used methods for orientation localization microscopy against the fundamental limits of measurement precision. Our analysis reveals optimal imaging methods for various experiment conditions and sample geometries. Interestingly, simple modifications to the standard fluorescence microscope exhibit superior performance in many imaging scenarios.

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Cited by in corpus (5)

• Single-molecule orientation localization microscopy I: fundamental limits
• Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes
• Resolving the Orientations of and Angular Separation between a Pair of Dipole Emitters
• Dipole-Spread Function Engineering for 6D Super-Resolution Microscopy
• Quantum limits to resolution and discrimination of spontaneous emission lifetimes