Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging
arXiv:2001.09232 · doi:10.1364/JOSAA.389217
Abstract
We investigate rotational diffusion of fluorescent molecules in angular potential wells, the excitation and subsequent emissions from these diffusing molecules, and the imaging of these emissions with high-NA aplanatic optical microscopes. Although dipole emissions only transmit six low-frequency angular components, we show that angular structured illumination can alias higher frequency angular components into the passband of the imaging system. We show that the number of measurable angular components is limited by the relationships between three time scales: the rotational diffusion time, the fluorescence decay time, and the acquisition time. We demonstrate our model by simulating a numerical phantom in the limits of fast angular diffusion, slow angular diffusion, and weak potentials.
22 pages, 5 figures
References in corpus (6)
- Super-resolution Imaging of the Fluorescent Dipole Assembly with Polarized Structured Illumination Microscopy
- Fundamental precision bounds for three-dimensional optical localization microscopy with Poisson statistics
- Efficient wide-field FLIM
- Spatio-angular fluorescence microscopy I. Basic theory
- Quantum limits for precisely estimating the orientation and wobble of dipole emitters
- Spatio-angular fluorescence microscopy II. Paraxial 4 imaging