Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect

Abstract

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morphology and biochemistry, respectively, that have pioneered numerous applications along their independent technological maturity over the past few decades. However, the distinct label-free contrasts are inherently complementary and difficult to integrate due to the use of different light-matter interactions. Here, we present a unified imaging scheme that realizes simultaneous and in-situ acquisition of MV-fingerprint contrasts of single cells in the framework of QPI utilizing the mid-infrared photothermal effect. The fully label-free and robust integration of subcellular morphology and biochemistry would have important implications, especially for studying complex and fragile biological phenomena such as drug delivery, cellular diseases and stem cell development, where long-time observation of unperturbed cells are needed under low phototoxicity.

References in corpus (5)

• High-resolution 3D refractive index microscopy of multiple-scattering samples from intensity images
• Time-multiplexed structured illumination using a DMD for optical diffraction tomography
• Bond-Selective Transient Phase Microscopy
• Quantitative phase imaging with molecular vibrational sensitivity
• Molecular contrast on phase-contrast microscope

Cited by in corpus (10)

• Adaptive dynamic range shift (ADRIFT) quantitative phase imaging
• Mid-infrared photothermal single-live-cell imaging beyond video rate
• Mid-infrared single-photon 3D imaging
• Bond-Selective Intensity Diffraction Tomography
• Far-field Super-resolution Chemical Microscopy
• Mid-infrared wide-field nanoscopy
• Bond-selective interferometric scattering microscopy
• Background-Suppressed High-Throughput Mid-Infrared Photothermal Microscopy via Pupil Engineering
• Vibrational Spectroscopic Detection of Single Virus by a Confocal Interferometric Mid-Infrared Photothermal Microscope
• Fluorescence-Enhanced Mid-Infrared Photothermal Microscopy