Elucidating the Origin of Heterogeneous Anomalous Diffusion in the Cytoplasm of Mammalian Cells

Abstract

Diffusion of tracer particles in the cytoplasm of mammalian cells is often anomalous with a marked heterogeneity even within individual particle trajectories. Despite considerable efforts, the mechanisms behind these observations have remained largely elusive. To tackle this problem, we performed extensive single-particle tracking experiments on quantum dots in the cytoplasm of living mammalian cells at varying conditions. Analyses of the trajectories reveal a strong, microtubule-dependent subdiffusion with antipersistent increments and a substantial heterogeneity. Furthermore, particles stochastically switch between different mobility states, most likely due to transient associations with the cytoskeleton-shaken endoplasmic reticulum network. Comparison to simulations highlight that all experimental observations can be fully described by an intermittent fractional Brownian motion, alternating between two states of different mobility.

References in corpus (14)

• Anomalous transport in the crowded world of biological cells
• Random Time-Scale Invariant Diffusion and Transport Coefficients
• Brownian yet non-Gaussian diffusion: from superstatistics to subordination of diffusing diffusivities
• "Diffusing diffusivity": A model for anomalous and "anomalous yet Brownian" diffusion
• Single particle tracking in systems showing anomalous diffusion: the role of weak ergodicity breaking
• Anomalous diffusion and ergodicity breaking in heterogeneous diffusion processes
• Scaled Brownian motion: a paradoxical process with a time dependent diffusivity for the description of anomalous diffusion
• Non-universal tracer diffusion in crowded media of non-inert obstacles
• Improved estimation of anomalous diffusion exponents in single particle tracking experiments
• The Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork
• Anomalous diffusion in time-fluctuating non-stationary diffusivity landscapes
• Superdiffusive motion of membrane-targeting C2 domains
• Heterogeneities Shape Passive Intracellular Transport
• Unravelling intermittent features in single particle trajectories by a local convex hull method

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• Superstatistical approach of the anomalous exponent for scaled Brownian motion
• Being Heterogeneous Is Advantageous: Extreme Brownian Non-Gaussian Searches
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• Diffusion of intrinsically disordered proteins within viscoelastic membraneless droplets
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• Absence of confinement and non-Boltzmann stationary states of fractional Brownian motion in shallow external potentials
• Dynamics of switching processes: general results and applications to intermittent active motion
• Learning minimal representations of stochastic processes with variational autoencoders
• Objective comparison of methods to decode anomalous diffusion
• Change-point detection in anomalous-diffusion trajectories utilising machine-learning-based uncertainty estimates
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• Variable-order fractional master equation and clustering of particles: non-uniform lysosome distribution
• Fractional and scaled Brownian motion on the sphere: The effects of long-time correlations on navigation strategies
• Unravelling Heterogeneous Transport of Endosomes
• Efficient recurrent neural network methods for anomalously diffusing single particle short and noisy trajectories
• Conditional entropic approach to nonequilibrium complex systems with weak fluctuation correlation
• Weak correlation between fluctuations in protein diffusion inside bacteria
• Super-resolved anomalous diffusion: deciphering the joint distribution of anomalous exponent and diffusion coefficient
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• Evaluating Gaussianity of heterogeneous fractional Brownian motion
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• Complete Decomposition of Anomalous Diffusion in Variable Speed Generalized Lévy Walks