Superdiffusive motion of membrane-targeting C2 domains

Abstract

Membrane targeting domains play crucial roles in the association of signalling molecules to the plasma membrane. For most peripheral proteins, the protein-to-membrane interaction is transient. After proteins dissociate from the membrane they have been observed to rebind following brief excursions in the bulk solution. Such membrane hops can have broad implications for the efficiency of reactions on membranes. We study the diffusion of membrane-targeting C2 domains using single-molecule tracking in supported lipid bilayers. The ensemble-averaged mean square displacement (MSD) exhibits superdiffusive behaviour. However, traditional time-averaged MSD analysis of individual trajectories remains linear and it does not reveal superdiffusion. Our observations are explained in terms of bulk excursions that introduce jumps with a heavy-tail distribution. These hopping events allow proteins to explore large areas in a short time. The experimental results are shown to be consistent with analytical models of bulk-mediated diffusion and numerical simulations.

References in corpus (4)

• Anomalous transport in the crowded world of biological cells
• Weak ergodicity breaking of receptor motion in living cells stemming from random diffusivity
• Development of anomalous diffusion among crowding proteins
• Nonergodisity of a time series obeying Lévy statistics

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• Infinite ergodic theory meets Boltzmann statistics
• Anomalous protein kinetics on low-fouling surfaces
• Non-normalizable quasi-equilibrium solution of the Fokker-Planck equation for nonconfining fields
• Two-dimensional Brownian motion with dependent components: turning angle analysis