Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations
arXiv:1802.08986 · doi:10.1364/OE.26.009866
Abstract
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and exploit the optical memory effect to enable fluorescence microscopy through a turbid layer. We demonstrate efficient reconstruction of micrometer-size fluorescent objects behind a scattering medium in epi-microscopy, and study the specificities of this imaging modality (magnification, field of view, resolution) as compared to traditional microscopy. Using a modified phase retrieval algorithm to reconstruct fluorescent objects from speckle images, we demonstrate robust reconstructions even in relatively low signal to noise conditions. This modality is particularly appropriate for imaging in biological media, which are known to exhibit relatively large optical memory ranges compatible with tens of micrometers size field of views, and large spectral bandwidths compatible with emission fluorescence spectra of tens of nanometers widths.
References in corpus (10)
- Measuring the Transmission Matrix in Optics : An Approach to the Study and Control of Light Propagation in Disordered Media
- Non-invasive real-time imaging through scattering layers and around corners via speckle correlations
- Translation correlations in anisotropically scattering media
- Noninvasive nonlinear imaging through strongly-scattering turbid layers
- Characterization of the angular memory effect of scattered light in biological tissues
- Single-shot diffraction-limited imaging through scattering layers via bispectrum analysis
- Imaging blood cells through scattering biological tissue using speckle scanning microscopy
- Focusing polychromatic light through strongly scattering media
- Direct Determination of Diffusion Properties of Random Media from Speckle Contrast
- Imaging through a thin scattering layer and jointly retrieving the point-spread-function using phase-diversity
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- Non-invasive light focusing in scattering media using speckle variance optimization
- Readout of fluorescence functional signals through highly scattering tissue
- Non-invasive Focusing Through Scattering Layers Using Speckle-Correlations
- Imaging of objects through a thin scattering layer using a spectrally and spatially separated reference
- Tracking moving objects through scattering media via speckle correlations
- Noninvasive linear fluorescence imaging through scattering media via wavefront shaping
- Deterministic Terahertz wave control in scattering media
- Non-invasive single-shot recovery of point-spread function of a memory effect based scattering imaging system
- Focusing large spectral bandwidths through scattering media
- Customizing the angular memory effect for scattering media
- Extrapolated speckle-correlation imaging
- Simultaneously improving multiple imaging parameters with scattering media