Readout of fluorescence functional signals through highly scattering tissue
arXiv:1906.02604 · doi:10.1038/s41566-020-0612-2
Abstract
Fluorescence is a powerful mean to probe information processing in the mammalian brain. However, neuronal tissues are highly heterogeneous and thus opaque to light. A wide set of non-invasive or invasive techniques for scattered light rejection, optical sectioning or localized excitation, have been developed, but non-invasive optical recording of activity through highly scattering layer beyond the ballistic regime is to date impossible. Here, we show that functional signals from fluorescent time-varying sources located below an highly scattering tissue can be retrieved efficiently, by exploiting matrix factorization algorithms to demix this information from low contrast fluorescence speckle patterns.
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- Methods, trade-offs and opportunities in high-speed optical microscopy for neural voltage imaging