Noninvasive linear fluorescence imaging through scattering media via wavefront shaping
arXiv:1904.02509 · doi:10.1364/OE.27.021778
Abstract
We demonstrate focusing and imaging through a scattering medium noninvasively by using wavefront shaping. Our concept is based on utilizing the spatial fluorescence contrast which naturally exists in the hidden target object. By scanning the angle of incidence of the illuminating laser beam and maximizing the variation of the detected fluorescence signal from the object, as measured by a bucket detector at the front of the scattering medium, we are able to generate a tightly focused excitation spot. Thereafter, an image is obtained by scanning the focus over the object. The requirements for applicability of the method are discussed.
10 pages, 5 figures
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- Model-based wavefront shaping microscopy
- Probing the position-dependent optical energy fluence rate in three-dimensional scattering samples