Refractive index tomography with structured illumination

Abstract

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically equivalent to a superposition of angled illuminations. It follows that raw acquisitions for standard SI-enhanced quantitative-phase images can be processed into complex electric-field maps describing sample diffraction under angled illuminations. Standard diffraction tomography (DT) computation can then be used to reconstruct the sample 3D RI distribution at sub-diffraction resolutions. We demonstrate this concept by using a SI-quantitative-phase imaging system to computationally reconstruct 3D RI distributions of human breast (MCF-7) and colorectal (HT-29) adenocarcinoma cells. Our experimental setup uses a spatial light modulator to generate structured patterns at the sample and collects angle-dependent sample diffraction using a common-path, off-axis interference configuration with no moving components. Furthermore, this technique holds promise for easy pairing with SI fluorescence microscopy, and important future extensions may include multimodal, sub-diffraction resolution, 3D RI and fluorescent visualizations.

arXiv comment19 total pages (including references), 8 figures

References in corpus (3)

• Wide-field, high-resolution Fourier ptychographic microscopy
• Time-multiplexed structured illumination using a DMD for optical diffraction tomography
• Hyperspectral optical diffraction tomography

Cited by in corpus (12)

• High-resolution 3D refractive index microscopy of multiple-scattering samples from intensity images
• Diffraction tomography with a deep image prior
• 3D correlative single-cell imaging utilizing fluorescence and refractive index tomography
• Computational structured illumination for high-content fluorescent and phase microscopy
• Review on methods of solving the refractive index-thickness coupling problem in digital holographic microscopy of biological cells
• Compensation of aberration and speckle noise in quantitative phase imaging using lateral shifting and spiral phase integration
• Far-field Super-resolution Chemical Microscopy
• Reconstructions of refractive index tomograms via discrete algebraic reconstruction technique
• Fourier synthesis optical diffraction tomography for kilohertz rate volumetric imaging
• High-speed multiview imaging approaching 4pi steradians using conic section mirrors: theoretical and practical considerations
• Model-based wavefront shaping microscopy
• Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction