Hyperspectral optical diffraction tomography

Abstract

Here, we present a novel microscopic technique for measuring wavelength-dependent three-dimensional (3-D) distributions of the refractive indices (RIs) of microscopic samples in the visible wavelengths. Employing 3-D quantitative phase microscopy techniques with a wavelength-swept source, 3-D RI tomograms were obtained in the range of 450 - 700 nm with a spectral resolution of a few nanometers. The capability of the technique was demonstrated by measuring the hyperspectral 3-D RI tomograms of polystyrene beads, human red blood cells, and hepatocytes. The results demonstrate the potential for label-free molecular specific 3-D tomography of biological samples.

References in corpus (1)

• 3-D refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood

Cited by in corpus (5)

• High-resolution 3D refractive index microscopy of multiple-scattering samples from intensity images
• Refractive index tomography with structured illumination
• The effects of spatiotemporal coherence on interferometric imaging
• Measurements of polarization-dependent angle-resolved light scattering from individual microscopic samples using Fourier transform light scattering
• Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction