Biomimetic wet-stable fibres via wet spinning and diacid-based crosslinking of collagen triple helices
arXiv:1509.05640 · doi:10.1016/j.polymer.2015.09.037
Abstract
One of the limitations of electrospun collagen as bone-like fibrous structure is the potential collagen triple helix denaturation in the fibre state and the corresponding inadequate wet stability even after crosslinking. Here, we have demonstrated the feasibility of accomplishing wet-stable fibres by wet spinning and diacid-based crosslinking of collagen triple helices, whereby fibre ability to act as bone-mimicking mineralisation system has also been explored. Circular dichroism (CD) demonstrated nearly complete triple helix retention in resulting wet-spun fibres, and the corresponding chemically crosslinked fibres successfully preserved their fibrous morphology following 1-week incubation in phosphate buffer solution (PBS). The presented novel diacid-based crosslinking route imparted superior tensile modulus and strength to the resulting fibres indicating that covalent functionalization of distant collagen molecules is unlikely to be accomplished by current state-of-the-art carbodiimide-based crosslinking. To mimic the constituents of natural bone extra cellular matrix (ECM), the crosslinked fibres were coated with carbonated hydroxyapatite (CHA) through biomimetic precipitation, resulting in an attractive biomaterial for guided bone regeneration (GBR), e.g. in bony defects of the maxillofacial region.
35 pages, 14 figures, 1 table, 1 scheme (manuscript accepted in "Polymer")
Cited by in corpus (6)
- Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model
- A Structurally Self-Assembled Peptide Nano-Architecture by One-Step Electrospinning
- In-situ crosslinked wet spun collagen triple helices with nanoscale-regulated ciprofloxacin release capability
- A Review of Multi-material and Composite Parts Production by Modified Additive Manufacturing Methods
- In silico modeling of the rheological properties of covalently crosslinked collagen triple helices
- Rotation-assisted wet-spinning of UV-cured gelatin fibres and nonwovens