Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model

Abstract

The design of exudate-managing wound dressings is an established route to accelerated healing, although such design remains a challenge from material and manufacturing standpoints. Aiming towards the clinical translation of knowledge gained in vitro with highly swollen rat tail collagen hydrogels, this study investigated the healing capability in a diabetic mouse wound model of telopeptide-free, protease-inhibiting collagen networks. 4 vinylbenzylation and UV irradiation of type I atelocollagen (AC) led to hydrogel networks with chemical and macroscopic properties comparable to previous collagen analogues, attributable to similar lysine content and dichroic properties. After 4 days in vitro, hydrogels induced nearly 50 RFU% reduction in matrix metalloproteinase (MMP)-9 activity, whilst showing less than 20 wt.-% weight loss. After 20 days in vivo, dry networks promoted 99% closure of 10x10 mm full thickness wounds and accelerated neodermal tissue formation compared to Mepilex. This collagen system can be equipped with multiple, customisable properties and functions key to personalised chronic wound care.

arXiv comment30 pages, 1 scheme, 6 Figures, 2 Supporting Figures

References in corpus (6)

• Photo-active collagen systems with controlled triple helix architecture
• Triple-helical collagen hydrogels via covalent aromatic functionalization with 1,3-Phenylenediacetic acid
• Biomimetic wet-stable fibres via wet spinning and diacid-based crosslinking of collagen triple helices
• Compositional and in Vitro Evaluation of Nonwoven Type I Collagen/Poly-dl-lactic Acid Scaffolds for Bone Regeneration
• Investigation into the potential use of Poly (vinyl alcohol)/Methylglyoxal fibres as antibacterial wound dressing components
• Structure-property-function relationships in triple helical collagen hydrogels

Cited by in corpus (4)

• A redox-responsive hyaluronic acid-based hydrogel for chronic wound management
• A UV-cured nanofibrous membrane of vinylbenzylated gelatin-poly(ε-caprolactone) dimethacrylate co-network by scalable free surface electrospinning
• Monomer-induced customisation of UV-cured atelocollagen hydrogel networks
• A hydroxamic acid-methacrylated collagen conjugate for the modulation of inflammation-related MMP upregulation