Intergenerational continuity of cell shape dynamics in Caulobacter crescentus
arXiv:1503.07147 · doi:10.1038/srep09155
Abstract
We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional widths, and medial curvatures from high accuracy measurements of cell contours. Moreover, these shape parameters are determined for over 250 cells across approximately 10000 total generations, which affords high statistical precision. Our data and model show that constriction is initiated early in the cell cycle and that its dynamics are controlled by the time scale of exponential longitudinal growth. Based on our extensive and detailed growth and contour data, we develop a minimal mechanical model that quantitatively accounts for the cell shape dynamics and suggests that the asymmetric location of the division plane reflects the distinct mechanical properties of the stalked and swarmer poles. Furthermore, we find that the asymmetry in the division plane location is inherited from the previous generation. We interpret these results in terms of the current molecular understanding of shape, growth, and division of C. crescentus
References in corpus (5)
- Direct measurement of cell wall stress-stiffening and turgor pressure in live bacterial cells
- Scaling laws governing stochastic growth and division of single bacterial cells
- Universality in stochastic exponential growth
- Dislocation-mediated growth of bacterial cell walls
- Phase resetting reveals network dynamics underlying a bacterial cell cycle