Networked Enzymatic Logic Gates with Filtering: New Theoretical Modeling Expressions and Their Experimental Application

Abstract

We report the first study of a network of connected enzyme-catalyzed reactions, with added chemical and enzymatic processes that incorporate the recently developed biochemical filtering steps into the functioning of this biocatalytic cascade. New theoretical expressions are derived to allow simple, few-parameter modeling of network components concatenated in such cascades, both with and without filtering. The derived expressions are tested against experimental data obtained for the realized network's responses, measured optically, to variations of its input chemicals' concentrations with and without filtering processes. We also describe how the present modeling approach captures and explains several observations and features identified in earlier studies of enzymatic processes when they were considered as potential network components for multi-step information/signal processing systems.

Keywords: binary AND; biocatalytic cascade; biochemical signals; multi-input biosensor

References in corpus (8)

• Enzymatic AND Logic Gates Operated Under Conditions Characteristic of Biomedical Applications
• Enzyme-Based Logic Analysis of Biomarkers at Physiological Concentrations: AND Gate with Double-Sigmoid "Filter" Response
• Biochemical Filter with Sigmoidal Response: Increasing the Complexity of Biomolecular Logic
• Enzymatic AND Logic Gate with Sigmoid Response Induced by Photochemically Controlled Oxidation of the Output
• Control of Noise in Chemical and Biochemical Information Processing
• Modularity of Biochemical Filtering for Inducing Sigmoid Response in Both Inputs in an Enzymatic AND Gate
• Bioinspired Associative Memory System Based on Enzymatic Cascades
• Realization of Associative Memory in an Enzymatic Process: Towards Biomolecular Networks with Learning and Unlearning Functionalities

Cited by in corpus (5)

• Kinetic Model for a Threshold Filter in an Enzymatic System for Bioanalytical and Biocomputing Applications
• Can bio-inspired information processing steps be realized as synthetic biochemical processes?
• Promises and Challenges in Continuous Tracking Utilizing Amino Acids in Skin Secretions for Active Multi-Factor Biometric Authentication for Cybersecurity
• Modeling and Modifying Response of Biochemical Processes for Biocomputing and Biosensing Signal Processing
• Theoretical Modeling Expressions for Networked Enzymatic Signal Processing Steps as Logic Gates Optimized by Filtering