How knots influence properties of proteins

Abstract

Molecular dynamics studies within a coarse-grained structure based model were used on two similar proteins belonging to the transcarbamylase family to probe the effects in the native structure of a knot. The first protein, N-acetylornithine transcarbamylase, contains no knot whereas human ormithine transcarbamylase contains a trefoil knot located deep within the sequence. In addition, we also analyzed a modified transferase with the knot removed by the appropriate change of a knot-making crossing of the protein chain. The studies of thermally- and mechanically-induced unfolding processes suggest a larger intrinsic stability of the protein with the knot.

23 pages, 12 figures

References in corpus (4)

• Tightening of knots in proteins
• Diffusion mechanisms of localised knots along a polymer
• Thermal unfolding of proteins
• Stretching of Proteins in the Entropic Limit

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