The role of microtubule movement in bidirectional organelle transport

Abstract

We study the role of microtubule movement in bidirectional organelle transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells serve as sensitive probes of motor induced, noisy cytoskeletal motions. Multiple peroxisomes move in unison over large time windows and show correlations with microtubule tip positions, indicating rapid microtubule fluctuations in the longitudinal direction. We report the first high-resolution measurement of longitudinal microtubule fluctuations performed by tracing such pairs of co-moving peroxisomes. The resulting picture shows that motor-dependent longitudinal microtubule oscillations contribute significantly to cargo movement along microtubules. Thus, contrary to the conventional view, organelle transport cannot be described solely in terms of cargo movement along stationary microtubule tracks, but instead includes a strong contribution from the movement of the tracks.

arXiv comment24 pages, 5 figures

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• Motility states in bidirectional cargo transport
• Environmental control of microtubule-based bidirectional cargo-transport
• The dynamics of cargo driven by molecular motors in the context of asymmetric simple exclusion processes
• Fluctuation effects in bidirectional cargo transport
• Heterogeneous model for superdiffusive movement of dense-core vesicles in C. elegans
• Cooperative Lattice Dynamics and Anomalous Fluctuations of Microtubules
• Jamming of molecular motors as a tool for transport cargos along microtubules
• A hopping mechanism for cargo transport by molecular motors in crowded microtubules