204 citations · 265 across the 8 of their papers we have counts for
8 papers
Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites
Helen L . Miller, Sonia Contera, Adam J. M. Wollman +5
Intercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery.…
Single-molecule techniques in biophysics: a review of the progress in methods and applications
Helen Miller, Zhaokun Zhou, Jack Shepherd +2
Single-molecule biophysics has transformed our understanding of the fundamental molecular processes involved in living biological systems, but also of the fascinating physics of li…
An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy
Adam J. M. Wollman, Helen Miller, Simon Foster +1
Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image anal…
Designing a single-molecule biophysics tool for characterizing DNA damage for techniques that kill infectious pathogens through DNA damage effects
Helen Miller, Adam J. M. Wollman, Mark C. Leake
Antibiotics such as the quinolones and fluoroquinolones kill bacterial pathogens ultimately through DNA damage. They target the essential type IIA topoisomerases in bacteria by sta…
Automated image segmentation and division plane detection in single live Staphylococcus aureus cells
Adam J. M. Wollman, Helen Miller, Simon Foster +1
Staphylococcus aureus is a coccal bacterium, which divides by binary fission. After division the cells remain attached giving rise to small clusters, with a characteristic 'bunch o…
Developing a new biophysical tool to combine magneto-optical tweezers with super-resolution fluorescence microscopy
Zhaokun Zhou, Helen Miller, Adam J. M. Wollman +1
We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse config…
Probing DNA interactions with proteins using a single-molecule toolbox: inside the cell, in a test tube, and in a computer
Adam J. M. Wollman, Helen Miller, Zhaokun Zhou +1
DNA-interacting proteins have roles multiple processes, many operating as molecular machines which undergo dynamic metastable transitions to bring about their biological function.…
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Helen Miller, Zhaokun Zhou, Adam J. M. Wollman +1
As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating…