4 papers · 1 filter
Competition for binding targets results in paradoxical effects for simultaneous activator and repressor action -- Extended Version
M. Ali Al-Radhawi, Krishna Manoj, Dhruv D. Jatkar +3
In the context of epigenetic transformations in cancer metastasis, a puzzling effect was recently discovered, in which the elimination (knock-out) of an activating regulatory eleme…
Multi-variable control to mitigate loads in CRISPRa networks: Extended Version
Krishna Manoj, Theodore W. Grunberg, Domitilla Del Vecchio
The discovery of CRISPR-mediated gene activation (CRISPRa) has transformed the way in which we perform genetic screening, bioproduction and therapeutics through its ability to scal…
Analysis of singularly perturbed stochastic chemical reaction networks motivated by applications to epigenetic cell memory
Simone Bruno, Felipe A. Campos, Yi Fu +2
Epigenetic cell memory, the inheritance of gene expression patterns across subsequent cell divisions, is a critical property of multi-cellular organisms. In recent work [10], a sub…
A Stein's Method Approach to the Linear Noise Approximation for Stationary Distributions of Chemical Reaction Networks
Theodore W. Grunberg, Domitilla Del Vecchio
Stochastic Chemical Reaction Networks are continuous time Markov chain models that describe the time evolution of the molecular counts of species interacting stochastically via dis…