33 citations · 242 across the 33 of their papers we have counts for
19 papers · 1 filter
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs
Lukas Spantzel, Iván Pérez, Thomas Heitkamp +4
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor. The receptor is activated by a small peptide ligand neurotensin. NTSR1 can be expressed in HEK cells by st…
Ligand-induced oligomerization of the human GPCR neurotensin receptor 1 monitored in living HEK293T cells
Anika Westphal, Hendrik Sielaff, Stefanie Reuter +3
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that can be expressed in HEK293T cells by stable transfection. Its ligand is a 13-amino-acid peptide that b…
Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET
Thomas Heitkamp, Reinhard Grisshammer, Michael Börsch
Neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that is important for signaling in the brain and the gut. Its agonist ligand neurotensin (NTS), a 13-amino-acid pepti…
Analyzing conformational changes in single FRET-labeled A1 parts of archaeal A1AO-ATP synthase
Hendrik Sielaff, Dhirendra Singh, Gerhard Grueber +1
ATP synthases utilize a proton motive force to synthesize ATP. In reverse, these membrane-embedded enzymes can also hydrolyze ATP to pump protons over the membrane. To prevent wast…
Binding of the immunomodulatory drug Bz-423 to mitochondrial FoF1-ATP synthase in living cells by FRET acceptor photobleaching
Ilka Starke, Kathryn M. Johnson, Jan Petersen +4
Bz-423 is a promising new drug for treatment of autoimmune diseases. This small molecule binds to subunit OSCP of the mitochondrial enzyme FoF1-ATP synthase and modulates its catal…
Observing single FoF1-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor
Thomas Heitkamp, Gabriele Deckers-Hebestreit, Michael Börsch
Adenosine triphosphate (ATP) is the universal chemical energy currency for cellular activities provided mainly by the membrane enzyme FoF1-ATP synthase in bacteria, chloroplasts an…