550 citations · 910 across the 4 of their papers we have counts for
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The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states
Jamieson A L Howard, Benjamin Ambrose, Mahmoud A S Abdelhamid +10
DNA helicases undergo conformational changes; however, their structural dynamics are poorly understood. Here, we study single molecules of superfamily 1A DNA helicase Rep, which un…
A new twist on PIFE: photoisomerisation-related fluorescence enhancement
Evelyn Ploetz, Benjamin Ambrose, Anders Barth +12
PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as…
The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices
Eitan Lerner, Benjamin Ambrose, Anders Barth +23
Single-molecule Förster resonance energy transfer (smFRET) has become a mainstream technique for probing biomolecular structural dynamics. The rapid and wide adoption of the techni…