31 citations · 31 across the 2 of their papers we have counts for
4 papers
The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states
Jamieson A L Howard, Benjamin Ambrose, Mahmoud A S Abdelhamid +10
DNA helicases undergo conformational changes; however, their structural dynamics are poorly understood. Here, we study single molecules of superfamily 1A DNA helicase Rep, which un…
A new twist on PIFE: photoisomerisation-related fluorescence enhancement
Evelyn Ploetz, Benjamin Ambrose, Anders Barth +12
PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as…
The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices
Eitan Lerner, Benjamin Ambrose, Anders Barth +23
Single-molecule Förster resonance energy transfer (smFRET) has become a mainstream technique for probing biomolecular structural dynamics. The rapid and wide adoption of the techni…
Precision and accuracy of single-molecule FRET measurements - a worldwide benchmark study
Björn Hellenkamp, Sonja Schmid, Olga Doroshenko +50
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However…