102 citations · 235 across the 19 of their papers we have counts for
21 papers
Engineering T7 RNA Polymerase for High-Purity In Vitro Transcription
Pauline Hermans, Youlia Serikova, José Castillo +2
In vitro transcription using bacteriophage T7 RNA polymerase (T7 RNAP) is the gold-standard platform for RNA production in both research and therapeutic applications. Despite its h…
Critical review of conformational B-cell epitope prediction methods
Gabriel Cia, Fabrizio Pucci, Marianne Rooman
Accurate in-silico prediction of conformational B-cell epitopes would lead to major improvements in disease diagnostics, drug design and vaccine development. A variety of computati…
AI challenges for predicting the impact of mutations on protein stability
Fabrizio Pucci, Martin Schwersensky, Marianne Rooman
Stability is a key ingredient of protein fitness and its modification through targeted mutations has applications in various fields such as protein engineering, drug design and del…
Modeling the molecular impact of SARS-CoV-2 infection on the renin-angiotensin system
Fabrizio Pucci, Philippe Bogaerts, Marianne Rooman
SARS-CoV-2 coronavirus infection is mediated by the binding of its spike protein to the angiotensin-converting enzyme 2 (ACE2), which plays a pivotal role in the renin-angiotensin…
Shedding light on the dark matter of the biomolecular structural universe: Progress in RNA 3D structure prediction
Fabrizio Pucci, Alexander Schug
Structured RNA plays many functionally relevant roles in molecular life. Structural information, while required to understand the functional cycles in detail, is challenging to gat…
Deciphering noise amplification and reduction in open chemical reaction networks
Fabrizio Pucci, Marianne Rooman
The impact of random fluctuations on the dynamical behavior a complex biological systems is a longstanding issue, whose understanding would shed light on the evolutionary pressure…