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FRET nanoscopy enables seamless imaging of molecular assemblies with sub-nanometer resolution
Jan-Hendrik Budde, Nicolaas van der Voort, Suren Felekyan +11
By circumventing the optical diffraction limit, super-resolved fluorescence microscopies enable the study of larger cellular structures and molecular assemblies. However, fluoresce…
Unraveling multi-state molecular dynamics in single-molecule FRET experiments -- Part II: Quantitative analysis of multi-state kinetic networks
Oleg Opanasyuk, Anders Barth, Thomas-Otavio Peulen +4
Single-molecule Förster Resonance Energy Transfer (smFRET) is ideally suited to resolve the dynamics of biomolecules. A significant challenge to date is capturing and quantifying t…
Unraveling multi-state molecular dynamics in single-molecule FRET experiments- Part I: Theory of FRET-Lines
Anders Barth, Oleg Opanasyuk, Thomas-Otavio Peulen +4
Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful approach to infor…