204 citations · 249 across the 11 of their papers we have counts for
5 papers · 1 filter
RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity
Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd +2
The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous recombination to resolve dou…
The End Restraint Method for Mechanically Perturbing Nucleic Acids in silico
Jack W Shepherd, Mark C Leake
Far from being a passive information store, the genome is a mechanically dynamic and diverse system in which torsion and tension fluctuate and combine to determine structure and he…
Investigating molecular crowding during cell division in budding yeast with FRET
Sarah Lecinski, Jack W Shepherd, Lewis Frame +3
Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in th…
PySTACHIO: Python Single-molecule TrAcking stoiCHiometry Intensity and simulatiOn, a flexible, extensible, beginner-friendly and optimized program for analysis of single-molecule microscopy
Jack W Shepherd, Ed J Higgins, Adam J M Wollman +1
As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routin…
Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes
Jack W Shepherd, Alex L Payne-Dwyer, Ji-Eun Lee +2
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Flu…