most citedLabel-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure?

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physics.optics2026

Femtosecond-to-millisecond holographic imaging of laser ablation dynamics

Shotaro Kawano, Keiichiro Toda, Miu Tamamitsu +3

Femtosecond laser ablation redistributes optically deposited energy across electronic, structural, mechanical, and thermal degrees of freedom over timescales from femtoseconds to m…

physics.optics2026

Pulse-to-pulse spectral phase characterization of mid-infrared pulses at megahertz rates

Zhihao Deng, Zicong Xu, Kazuki Hashimoto +3

Pulse-resolved spectral phase measurement of mid-infrared (MIR) pulses is essential for many applications, from precise waveform control to ultrafast quantum optics. However, conve…

physics.optics2026

A unified framework for sensitivity comparison across stimulated Raman, stimulated Raman photothermal, and mid-infrared photothermal microscopy

Keiichiro Toda, Takuro Ideguchi

Label-free vibrational microscopy based on Raman scattering and mid-infrared (MIR) absorption has advanced biological imaging through the development of diverse high-sensitivity mo…

physics.optics2026

Mid-infrared photothermal imaging flow cytometry

Yusei Sugawara, Keiichiro Toda, Genki Ishigane +2

Imaging flow cytometry (IFC) enables high-throughput single-cell analysis but largely relies on fluorescence labeling to obtain molecular specificity. Label-free vibrational imagin…

physics.optics2026

Video-rate volumetric chemical imaging via mid-infrared photothermal optical diffraction tomography

Masato Fukushima, Keiichiro Toda, Yusei Sugawara +2

Label-free vibrational microscopy provides chemically specific access to cellular structure, yet quantitative volumetric chemical dynamics in living cells remain largely inaccessib…

physics.optics2025

Bidirectional quantitative scattering microscopy

Kohki Horie, Keiichiro Toda, Takuma Nakamura +1

Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques and are widely used for biomedical applications. Ea…