Forced-unfolding and force-quench refolding of RNA hairpins

Abstract

Using coarse-grained model we have explored forced-unfolding of RNA hairpin as a function of $f_S$ and the loading rate ($r_f$). The simulations and theoretical analysis have been done without and with the handles that are explicitly modeled by semiflexible polymer chains. The mechanisms and time scales for denaturation by temperature jump and mechanical unfolding are vastly different. The directed perturbation of the native state by $f_S$ results in a sequential unfolding of the hairpin starting from their ends whereas thermal denaturation occurs stochastically. From the dependence of the unfolding rates on $r_f$ and $f_S$ we show that the position of the unfolding transition state (TS) is not a constant but moves dramatically as either $r_f$ or $f_S$ is changed. The TS movements are interpreted by adopting the Hammond postulate for forced-unfolding. Forced-unfolding simulations of RNA, with handles attached to the two ends, show that the value of the unfolding force increases (especially at high pulling speeds) as the length of the handles increases. The pathways for refolding of RNA from stretched initial conformation, upon quenching $f_S$ to the quench force $f_Q$, are highly heterogeneous. The refolding times, upon force quench, are at least an order of magnitude greater than those obtained by temperature quench. The long $f_Q$-dependent refolding times starting from fully stretched states are analyzed using a model that accounts for the microscopic steps in the rate limiting step which involves the trans to gauche transitions of the dihedral angles in the GAAA tetraloop. The simulations with explicit molecular model for the handles show that the dynamics of force-quench refolding is strongly dependent on the interplay of their contour length and the persistence length, and the RNA persistence length.

42 pages, 15 figures, Biophys. J. (in press)

Cited by in corpus (16)

• Dynamics of allosteric transitions in GroEL
• Mechanical unfolding of RNA: From hairpins to structures with internal multiloops
• From mechanical folding trajectories to intrinsic energy landscapes of biopolymers
• Measuring the energy landscape roughness and the transition state location of biomolecules using single molecule mechanical unfolding experiments
• Force Dependent Hopping Rates of RNA Hairpins can be Estimated from Accurate Measurement of the Folding Landscapes
• Predicting 3D structure and stability of RNA pseudoknots in monovalent and divalent ion solutions
• Changing the mechanical unfolding pathway of FnIII10 by tuning the pulling strength
• Dynamic force spectroscopy of DNA hairpins. I. Force kinetics and free energy landscapes
• Heterogeneous morphology and dynamics of polyelectrolyte brush condensates in trivalent counterion solution
• Crowding effects on the mechanical stability and unfolding pathways of Ubiquitin
• Dynamic force spectroscopy of DNA hairpins. II. Irreversibility and dissipation
• The role of internal chain dynamics on the rupture kinetics of adhesive contacts
• RNA under Tension: Folding Landscapes, Kinetic Partitioning Mechanism, and Molecular Tensegrity
• Force Dependence of Proteins' Transition State Position and the Bell-Evans Model
• Extracting folding landscape characteristics of biomolecules using mechanical forces
• Pathways and kinetic barriers in mechanical unfolding and refolding of RNA and proteins