Elastic properties of grafted microtubules

Abstract

We use single-particle tracking to study the elastic properties of single microtubules grafted to a substrate. Thermal fluctuations of the free microtubule's end are recorded, in order to measure position distribution functions from which we calculate the persistence length of microtubules with contour lengths between 2.6 and 48 micrometers. We find the persistence length to vary by more than a factor of 20 over the total range of contour lengths. Our results support the hypothesis that shearing between protofilaments contributes significantly to the mechanics of microtubules.

9 pages, 3 figures

References in corpus (7)

• Radial distribution function of semiflexible polymers
• Force-Extension Relation and Plateau Modulus for Wormlike Chains
• Kinks, rings, and rackets in filamentous structures
• Entropic forces generated by grafted semiflexible polymers
• Depinning of semiflexible polymers in (1+1) dimensions
• Transverse fluctuations of grafted polymers
• Color suppressed contributions to the decay modes B_{d,s} -> D_{s,d} D_{s,d}, B_{d,s} -> D_{s,d} D^*_{s,d}, and B_{d,s} -> D^*_{s,d} D^*_{s,d}

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