Subcellular Metabolic Tracking Using Fluorescent Nanodiamonds Relaxometry
arXiv:2512.17931 · doi:10.1002/adfm.202527416
Abstract
Fluorescent nanodiamonds (FNDs) relaxometry holds promising future for advancement of high spatiotemporal resolution metabolic imaging technology. In this study, we demonstrate a simultaneous integration of spatial position tracking with FND relaxometry to characterize the temporal dynamics of metabolic processes, thereby enhancing the capability to monitor cellular activities over time. To enable targeted metabolic probing in living cells, FNDs were surface-functionalized to achieve specific localization within key organelles, including the nucleus and mitochondria. This strategy not only facilitates subcellular-level metabolic monitoring but also allows for direct comparison between intra- and extranuclear microenvironments within the same living cell, offering substantial potential for elucidating the spatial and functional heterogeneity of cellular metabolism.
arXiv comment30 pages, 5 figures
References in corpus (6)
- Nanometer scale quantum thermometry in a living cell
- Spin relaxometry of single nitrogen-vacancy defects in diamond nanocrystals for magnetic noise sensing
- Single-molecule Scale Magnetic Resonance Spectroscopy using Nitrogen-Vacancy Centers in Diamond
- Nanoscale Dynamic Readout of a Chemical Redox Process Using Radicals Coupled with Nitrogen-Vacancy Centers in Nanodiamonds
- In situ electron paramagnetic resonance spectroscopy using single nanodiamond sensors
- Long Spin Relaxation Times in CVD-Grown Nanodiamonds