Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological specimens

Abstract

Traditional optical tweezers techniques often rely on high-power continuous wave (CW) lasers, which can introduce unwanted thermal effects and photodamage to delicate samples. To overcome these limitations, we demonstrate femtosecond laser assisted selective holding with ultra-low power (FLASH-UP). We find that the FLASH-UP exhibits a five times greater trap stiffness than CW-OT, and can trap at lower intensities. Furthermore, we demonstrate OT of different pathogenic bacteria species and find that FLASH-UP does not impact cell motility. These results pave the way for applications in sorting, bio-sensing, in vivo cell manipulation and single cell analysis.

References in corpus (3)

• Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
• Dynamic radiation force of a pulsed Gaussian beam acting on a Rayleigh dielectric sphere
• OpTiDDM (Optical Tweezers integrating Differential Dynamic Microscopy) maps the spatiotemporal propagation of nonlinear stresses in polymer blends and composites

Cited by in corpus (2)

• Harnessing ultrafast optical pulses for 3D microfabrication by selective tweezing and immobilization of colloidal particles in an integrated system
• Selective Tweezing and Immobilization of Colloids for Dexterous Manipulation of Biological Materials