Role of particle local curvature in cellular wrapping

Abstract

Cellular uptake through the lipid membranes plays an important role in adsorbing nutrients and fighting infection and can be used for drug delivery and nanomedicine developments. Endocytosis is one of the known pathways of the cellular uptake which associate with elastic deformation of the membrane wrapping around the foreign particle. The deformability of the membrane itself is strongly regulated by the presence of a cortical cytoskeleton placed underneath the membrane. It has been shown that size, shape, and orientation of the particles influence on their entry into the cell. Here, we study the role of particle local curvature in the cellular uptake by investigating the wrapping of an elastic membrane around a long cylindrical object with an elliptical cross section. The membrane itself is adhered to a substrate mimicking the cytoskeleton. Membrane wrapping proceeds differently whether the initial contact occurs at the particle's highly curved tip (prolate) or along its long side (oblate). We obtain a wrapping phase diagram as a function of the membrane-cytoskeleton and the membrane-target adhesion energy, which includes three distinct regimes of engulfment(unwrapped, partially wrapped, and fully wrapped), separated by two phase transitions. We also provide analytical expressions for the boundary between the different regimes which confirm that the transitions strongly depend on the orientation and aspect ratio of the particle.

References in corpus (12)

• Efficient tunable generic model for fluid bilayer membranes
• Elastic deformation of a fluid membrane upon colloid binding
• Solvent free model for self-assembling fluid bilayer membranes: Stabilization of the fluid phase based on broad attractive tail potentials
• The mechanism of phagocytosis: two stages of engulfment
• Wrapping of ellipsoidal nano-particles by fluid membranes
• Target shape dependence in a simple model of receptor-mediated endocytosis and phagocytosis
• Interface mediated interactions between particles -- a geometrical approach
• Indirect interactions of membrane-adsorbed cylinders
• Balancing torques in membrane-mediated interactions: Exact results and numerical illustrations
• Model for probing membrane-cortex adhesion by micropipette aspiration and fluctuation spectroscopy
• Particle self-assembly on soft elastic shells
• Receptor-Mediated Endocytosis of a Cylindrical Nanoparticle in the Presence of Cytoskeleton Substrate

Cited by in corpus (1)

• Entropy-Driven Initiation and Cytoskeletal Viscoelasticity in Endocytosis: An Onsager Variational Framework