DNA origami
arXiv:2104.15016 · doi:10.1038/s43586-020-00009-8
Abstract
Biological materials are self-assembled with near-atomic precision in living cells, whereas synthetic 3D structures generally lack such precision and controllability. Recently, DNA nanotechnology, especially DNA origami technology, has been useful in the bottom-up fabrication of well-defined nanostructures ranging from tens of nanometres to sub-micrometres. In this Primer, we summarize the methodologies of DNA origami technology, including origami design, synthesis, functionalization and characterization. We highlight applications of origami structures in nanofabrication, nanophotonics and nanoelectronics, catalysis, computation, molecular machines, bioimaging, drug delivery and biophysics. We identify challenges for the field, including size limits, stability issues and the scale of production, and discuss their possible solutions. We further provide an outlook on next-generation DNA origami techniques that will allow in vivo synthesis and multiscale manufacturing.
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- Recent advances in DNA origami-engineered nanomaterials and applications
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- Design rules for controlling active topological defects
- Mechanical Metamaterials Fabricated from Self-assembly: A Perspective
- Tiling a tubule: How increasing complexity improves the yield of self-limited assembly
- On the origin of chirality in plasmonic meta-molecules
- Probing the Theoretical and Computational Limits of Dissipative Design
- Hairygami: Analysis of DNA Nanostructures' Conformational Change Driven by Functionalizable Overhangs
- Characterizing the free-energy landscapes of DNA origamis
- Mapping molecular complexes with Super-Resolution Microscopy and Single-Particle Analysis
- Modulating lipid membrane morphology by dynamic DNA origami networks
- Light-harvesting enhanced by quantum ratchet states
- Raman Enhancement in Bowtie-Shaped Aperture-Particle Hybrid Nanostructures Fabricated with DNA-Assisted Lithography
- Giant Purcell broadening and Lamb shift for DNA-assembled near-infrared quantum emitters
- Designing active colloidal folders
- Hotter isn't faster for a melting RNA hairpin
- The free-energy landscape of a mechanically bistable DNA origami
- Coupled Schrödinger equations as a model of interchain excitation transport in the DNA
- Leveraging the Versatility of DNA Origami and Electrochemistry for New Sensing Modalities
- Configuration space partitioning in tilings of a bounded region of the plane
- Coarse-grained models for phase separation in DNA-based fluids
- Tilings of a bounded region of the plane by maximal one-dimensional tiles