The free-energy landscape of a mechanically bistable DNA origami

Abstract

Molecular simulations using coarse-grained models allow the structure, dynamics and mechanics of DNA origamis to be comprehensively characterized. Here, we focus on the free-energy landscape of a jointed DNA origami that has been designed to exhibit two mechanically stable states and for which a bistable landscape has been inferred from ensembles of structures visualized by electron microscopy. Surprisingly, simulations using the oxDNA model predict that the defect-free origami has a single free-energy minimum. The expected second state is not stable because the hinge joints do not simply allow free angular motion but instead lead to increasing free-energetic penalties as the joint angles relevant to the second state are approached. This raises interesting questions about the cause of this difference between simulations and experiment, such as how assembly defects might affect the ensemble of structures observed experimentally.

main: 7 pages, 6 figures; supplementary material: 6 pages, 8 figures

References in corpus (6)

• DNA origami
• Introducing Improved Structural Properties and Salt Dependence into a Coarse-Grained Model of DNA
• Sequence-dependent thermodynamics of a coarse-grained DNA model
• Computing the elastic mechanical properties of rod-like DNA nanostructures
• Characterizing the free-energy landscapes of DNA origamis
• Measuring internal forces in single-stranded DNA: Application to a DNA force clamp